SCHEDULE 4 ANALYTICAL METHODOLOGY

TABLE A1: PARAMETERS FOR WHICH, SUBJECT TO REGULATION 16(7), METHODS OF ANALYSIS ARE PRESCRIBED

(1) (2)
Parameter Method
Clostridium perfringens (including spores) Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar* at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
Coliform bacteria ISO 9308-1
Colony count 22°C-enumeration of culturable microorganisms PrEN ISO 6222
Colony count 37°C-enumeration of culturable microoganisms prEN ISO 6222
Enterococci ISO 7899-2
Escherichia coli (E. coli) ISO 9308-1
*The composition of m-CP agar is:
Basal medium
Tryptose 30.0g
Yeast extract 20.0g
Sucrose 5.0g
L-cysteine hydrochloride 1.0g
MgSO4.7H2O 0.1g
Bromocresol purple 40.0mg
Agar 15.0g
Water 1,000.0ml
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D-cycloserine 400.0mg
Polymyxine-B sulphate 25.0mg
Indoxyl-[beta]-D-glucoside 60.0mg
to be dissolved in 8ml sterile water before addition
Filter-sterilised 0.5% phenolphthalein diphosphate solution 20.0ml
Filter-sterilised 4.5% FeCl3.6H2O 2.0ml

TABLE A2: PARAMETERS IN RELATION TO WHICH METHODS OF ANALYSIS MUST SATISFY PRESCRIBED CHARACTERISTICS

(1) (2) (3) (4)
Parameters Trueness % of prescribed concentration or value or specification Precision % of prescribed concentration or value or specification Limit of detection % of prescribed concentration or value or specification
Aluminium 10 10 10
Ammonium 10 10 10
Antimony 25 25 25
Arsenic 10 10 10
Benzene 25 25 25
Benzo(a)pyrene 25 25 25
Boron 10 10 10
Bromate 25 25 25
Cadmium 10 10 10
Chloride 10 10 10
Chromium 10 10 10
Colour 10 10 10
Conductivity 10 10 10
Copper 10 10 10
Cyanide (i) 10 10 10
1,2-dichloroethane 25 25 10
Fluoride 10 10 10
Iron 10 10 10
Lead 10 10 10
Manganese 10 10 10
Mercury 20 10 20
Nickel 10 10 10
Nitrate 10 10 10
Nitrite 10 10 10
Pesticides and related products (ii) 25 25 25
Polycyclic aromatic hydrocarbons (iii) 25 25 25
Selenium 10 10 10
Sodium 10 10 10
Sulphate 10 10 10
Tetrachloroethene (iv) 25 25 10
Tetrachloromethane 20 20 20
Trichloroethene (iv) 25 25 10
Trihalomethanes: 25 25 10
Total (iii)
Turbidity (v) 10 10 10
Turbidity (vi) 25 25 25

Notes:

(i)

The method of analysis should determine total cyanide in all forms.

(ii)

The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.

(iii)

The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1.

(iv)

The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1.

(v)

The performance characteristics apply to the prescribed value of 4 NTU.

(vi)

The performance characteristics apply to the specification of 1 NTU for water leaving treatment works.