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    1.    The reference methods for the analysis and testing of dairy products in order to ascertain if they meet the requirements specified in Schedules 3, 4, 5 and 6 shall be those specified in the following paragraphs of this Schedule.

    2.    in relation to raw milk and heat-treated milk, the methods shall be those specified in Commission Decision 91/180/EEC^[32] for the following—


(a)  enumeration of micro-organisms—plate count at 30°C;


(b)  enumeration of somatic cells in cows' milk;


(c)  determination of phosphatase activity in cows' milk;


(d)  determination of peroxidase activity in cow's milk;


(e)  detection of pathogenic micro-organisms;


(f)  enumeration of coliforms—colony count at 30°C; and


(g)  enumeration of micro-organisms—plate count at 21°C.

    3.    In relation to milk-based products, the test for enumeration of micro-organisms—plate count at 30°C shall be the method specified in IDF Standard 100B: 1991; Milk and milk products—micro-organisms—colony count at 30°C.

    4.    In relation to milk-based products, the test for enumeration of coliforms shall be the method specified in ISO 5541 Part 2 1986: Milk and milk products—Part 2; Most probable number technique at 30°C.

    5.    In relation to the following tests, the methods for the analysis and testing shall be those specified below—

Test	Method
(a) Enumeration of Staphylococcus aureus	Provisional IDF Standard 145: 1990; Milk and milk-based products. Enumeration of Staphylococcus aureus in products other than dried milk-Colony count technique at 37°C.
(b) Enumeration of Stapjylococcus aureus in powdered milk	Provisional IDF Standard 138: 1986; Dried Milk. Enumeration of Staphylococcus aureus. Colony count technique at 37°C.
(c) Detection of Salmonella spp	ISO 6597: 1993; Microbiology— General guidance on methods for the detection of Salmonella and IDF Standard 93A: 1985; sample preparation.
(d) Detection of Listeria monocytogenes	ISO 10560: 1993; Milk and milk productgs—Detection of Listeria monocytogenes.
(e) Enumeration of Escherichia coli in milk-based products	Provisional IDF standard 170: 1994. Milk and milk products—Enumeration of presumptive Escherichia coli content by: (i)  Most probable number technique. (ii)  most probable number technique with use of 4-methylumbelliferyl -β-D-glucuronide (MUG). (iii)  Colony count technique at 44°C using membranes.
(f) Detection of phosphatase (Residual) in cream	AOAC official method 950.41: Official Methods of Analysis, 16th edition: 1995.
(g) Detection of phosphatase (Reactivated and Residual) in cream	AOAC official method 965.27: Official Methods of Analysis, 16th edition: 1995.

    6.    any other internationally accepted analysis and test method as referred to in Article 20(1) of Council Directive 92/46 shall be recognised as a reference method.

    7.    In this Schedule—
"IDF standard" means a standard of the International Dairy Federation;


"ISO" means a standard of the International Organisation for Standardisation; and


"AOAC" means the Association of Official Analytical Chemists.

[32] OJ NO. L93, 13.4.91, p.1. back